entomopathogenic nematodes from the genera Steinernema are thought to be very patchy distributed in the experimental field. If this is true, this could explain that after a successful pilot study, no EPN´s are found in the analysis. This hypothesis is contradictory with the results Mráček et al. [12] found, were EPN occurrence was more ubiquitous than patchy. However, the study of Vladimír Půža et al. [17] found that patchiness of EPN’s differed per species. In future nematode analysis in the same field, patchiness and distribution of Steinernema could be determined. Therefore, more samples have to be taken, preferably also in other strips, apart from strip 1 and 6. The proposed follow-up study is beyond the scope of this research.

Heterorhabditis was not found in the pilot study, nor in the final analysis, therefore this genera is assumed not to occur in the studied field.

Patchiness

Similar to Steinernema, Filenchus group III and Diphtherophora were assumed to occur very patchy. This assumption is made, for their presence was detected, but only in a few plots in the field.

Species composition

No difference was found between Mix and No-mix treatments within strips. Although the plots differed in species composition, nematode abundances were not significantly influenced by this. This was possibly due to the fact that the experiment only started in 2014 and differences in soil condition, soil biota, fertility, etc. (if there were any) didn’t have the time to evolve yet. Expected is an increase in differences between Mix and No-mix plots over time. This is because those treatments (Mix as more diverse and No-mix as less diverse) will be implemented on every crop sown in the future on these plots.

Strip 6 had a more diverse Mix treatment than Strip 1 (as indicated in table 1). Effects of this more diverse grass-clover mixture are assumed to be negligible, for the difference between Mix and No-mix was insignificant and the sown species mixture of strip 6 had less time to establish than strip 1.

Total nematodes

The data on total nematode abundance obtained from counting and performing a Q-PCR differed with approximately a factor 10, the question rises whether both data-sets are representing reality. In this study,