Opioid abuse

You are free to propose whatever type of study you wish (as long as it is relevant to the behavioral sciences), but If you are thinking of using this proposed research for your capstone course you may want to ask a question that can be feasibly carried out here. That means you may want to avoid research designs that would require the use of protected populations such as children, prisoners, the elderly, mentally disabled individuals, etc. (see the IRB guidelines for more details on this). The IRB will also not allow questions about illicit drug use or other illegal behaviors. In the capstone course you will be restricted to studies that can be carried out in 12 weeks available and you will be responsible for any monetary costs associated with the study, so keep these in mind.

For this assignment you should answer the following using complete sentences:

What is the research question your study seeks to answer?
A good research question must be specific so that it can be answered with a research project. For example, “what causes depression?” is too broad and could not be answered in a study. “Is sunlight exposure related to depression?” would be a good research question because it is specific enough to be answered in a study. For this course, your research question should focus on the relationship between only two variables related to psychology or the behavioral sciences.

Be careful with the language you use in your research question. Terms like “effects”, “impacts”, “leads to”, etc. would all imply a causal relationship, which would only be appropriate if your study employs an experimental design. If your study is nonexperimental, your research question should instead use terms like “relationship”, “correlation”, “is related to”, “predicts”, “is associated with” etc.

What is the hypothesis for your study?
A hypothesis indicates the specific predicted relationship between the variables in a study. If you are investigating a correlation, indicate the predicted strength and direction of the relationship. If you are performing an experiment indicate the expected causal relationship.

What are the variables you will explore in your research question?
A variable is any measurable event, situation, behavior, or individual characteristic that varies (has at least two values). In your study you will be looking at a relationship (either causal or correlational, depending on your research design) between two variables.

Sample Solution

LC analysis
Baseline blood samples (day 0) collected from patients reporting no antimalarial intake prior to the study were screened for the presence of five antimalarial drugs such as CQ, SDX, PYR, QN and MQ using a modified HPLC method(Blessborn et al. 2010) . The level of partner drug (SDX) of AS+SP was also determined on day 7. Extraction of the standard drugs (CQ, QN, SDX, PYR and MQ; Sigma Aldrich, USA), blank whole blood spot (control sample) and each of the collected samples were carried out according to the protocol of Blessborn et al., 2010, with slight modification. This involved the use of multi-mode solid phase extraction column (M-M SPE, Biotage, USA) and elution of the samples by methanol:triethylamine (97:3 v/v) mixture. Eluates were dried under a gentle stream of air at 70”C and were then dissolved in 100 ”l of methanol:HCl (0.01 M) 10:90 v/v. Twenty microliter (20 ”l) of each of these standards and samples were injected into the HPLC system. HPLC was performed on a Hitachi gradient system equipped with binary pump (Model L-2100/2130) and multi wavelength UV detector (Model L-2420 UV-VIS). Analytes extracted from the M-M SPE column were analyzed using two different mobile phases (A) acetonitrile:ammonium formate (20 mM in 1% formic acid) (5:95 v/v) and (B) acetonitrile:ammonium formate (10 mM in 1% formic acid) (80:20 v/v) and were run according to previously described gradient program(Blessborn et al. 2010). The compounds were analyzed on a Tosoh ” 5 ”m C18 (150 mm ” 2 mm) column protected by a precolumn security guard C8 (8mm x2 mm) (Tosoh Bioscience, PA). The UV detector was monitored at 280nm. Data acquisition and quantification were performed using HystarTM and Dat

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