efore any inspecting or information assortment, a pilot search to find a scope of pony chestnut trees under various fake light powers was executed. No thought was given to some other factors like size or age. 30 trees were settled on. The trees were all in Bristol, Joined Realm and ran in area from Durdham Downs down into Clifton Town. The distance between the farthest trees was 1.7 miles. Each tree was given a number and its area was recorded utilizing Google MapsTM. The trees chose were across a scope of night light, from totally dim by road lighting to being encircled by road lighting.

2.2 Handout Assortment

To precisely report which trees the leaves were from, they were gathered prior to gathering the light information. Over a time of seven days, visits to the 30 foreordained trees were made. A pony chestnut tree leaf comprises of five to seven flyers. To keep up with consistency, the focal flyer was taken from each leaf and cut at the foundation of the handout utilizing scissors. It was this handout that was saved for later investigation. At each tree, ten pamphlets were gathered. To choose which pamphlets to take, it was resolved which flyer was least on the outside of the tree. The nine excess handouts were then gathered from around the underlying pamphlet inside a 30cm width. When the pamphlets from one tree had been gathered, they were placed into a sack which was named with the tree number. A photo was then taken remaining under the tree at the point the leaves were gathered from, holding the numbered pack.

The leaves were taken into the lab around the same time they were gathered and captured utilizing a Standard EOS M3 camera (Ordinance, Joined Realm). This was to keep the leaf in the manner it was found in the event that any excavators that were as yet alive kept on eating the leaf whenever it had been taken out from the tree. They were captured by mounting the camera on a stand and keeping the camera settings predictable so the photos would be all of a similar quality when it came to the examination. The flyers were put on a white foundation with the tree number sticker close to it so they could be ordered accurately once the photos were all taken. There was likewise a 30cm ruler in shot to permit a scale bar to be utilized when it came to examining the pamphlets.