Sample Solution
“The Fireman” by Rick Bass
In “The Fireman” by Rick Base, we glimpse inside the mind of an average person, who appears to be just that, very normal. Kirby is a volunteer fire fighter, so in addition to receiving no pay, he also has the perpetual intrusions this sort of job brings into his personal life. Kirby and Mary Ann`s marriage is a good marriage. They both stand on the other side of the miracle. Their marriage was bad, perhaps even rotting, but then it got better. He – the fireman, Kirby, knows what the reason is, that every time they have an argument, the dispatcher`s call sounds, and he must run and disappear into the flames, he is the captain, and while he is gone, his wife, Mary Ann, reorders her priorities, thinks of the children, and worries for him. Her blood cools, as does his. It seems that the dispatcher`s call is always saving them. Their marriage settles in and strengthens, afterward, like some healthy, living, supple thing.
ntegration is an irreversible activity done by integrase, the viral protein that introduces the viral ds-DNA into the cell’s chromatin. The integration results in forming small gaps in the chromatin because of integrase enzymatic activity are fixed by cellular proteins, and the viral DNA is at long last incorporated into the cellular genome. However, such intermediates like reversed-transcribed dsDNA are detected in linear or circular isoforms in the nucleus and the cytoplasm (Wu, 2004). Notwithstanding, unintegrated viral DNA has been shown to cause the depletion of major histocompatibility complex and viral receptors, and mediate expression of viral regulatory proteins the inactivity of intermediates like that is still debatable (Sloan et al., 2010, 2011).
The cellular transcription machinery is an important factor that the synthesis of full-length HIV genomic RNA depends on. Spliced transcribed HIV-RNA and shorter mRNA molecules are transported to the cytoplasm through atomic pores in a similar way as the cell mRNA (Cullen, 2003). The synthesis of viral proteins is done in a way which exploits the mechanism of cellular translation. Viral accessory protein, such as Tat, is synthesized and accumulates in the cell cytoplasm, transported to the nuclease, and expands HIV RNA translation (Zucker et al., 2001; Romani et al., 2010). Another viral accessory protein is called Rev that is also synthesized in the cytoplasm and then transported to the nucleus, where it mediates the transportation of full-length HIV RNA to the cytoplasm (Cullen, 2003).
Once the viral RNA and polyproteins have accumulated in the cytoplasm followed by viral particle formation and then full-length non-spliced HIV RNA is encapsidated and sprouted from the cell. The bone marrow stromal cell antigen 2/tetherin, is a cellular restriction factor that is responsible for the inhibition of the capacity of newly formed viruses to reinfect (Andrew and Strebel, 2010; Evans et al., 2010). HIV has Vpu, an accessory protein, that is able to work against this cellular restriction mechanism. Once the viral molecule is budded, it is immature and non-infectious. Maturation of the viral protease enzyme is mediated by itself and is in charge of the cleavage of capsid proteins, and renders the in
