er 2nd, 2018, the first lab session that the unknowns were handled, a Gram stain was performed on the mixed culture to observe morphology. Under the microscope, both Gram positive and Gram negative bacteria were present. The Gram positive organisms were coccus-shaped in grape-like clusters. The Gram negative organisms were rod-shaped.

One October 9th, 2018, the bacterial growth on the four plates were observed. The TSA plates at room temperature and at 37 degrees Celsius both had decent amounts of bacterial growth on them. On the room temperature TSA plate, one type of bacterial colony was about 3 millimeters in diameter, round-shaped, yellow, fuzzy, and flat. The other colony on the room temperature TSA plate was about 1.5 millimeters in diameter, circular, and off-white, with slight elevation and a defined edge. On the 37 degrees Celsius TSA plate, one colony was round and yellow, with cloudy edges and smooth elevation. The other colony was round and off-white, with defined edges and smooth elevation. The MSA plate was growth positive, and it appeared to only have one type of bacteria on it. The plate turned yellow in the areas where the bacteria grew, and the colonies were off-white, circular, and smooth, with slight elevation and defined edges. EMB plates select for Gram negative bacteria, and the bacterial colonies that grew on the plate were dark pink dots surrounded by fuzz with no green metallic sheen. During this lab session, the two bacteria were isolated through isolation streaks on two separate TSA plates that were then incubated at 37 degrees Celsius.

On October 23rd, 2018, the isolated Gram